Shocked by the ordeal, Neville sits down for a moment in his laboratory. Neville and the alpha male both stare each other down Neville apologizes to the Darkseekers the alpha male accepts his apology, and the Darkseekers leave. Neville puts his gun down and returns the female. Neville realizes that the alpha male is identifying the female upon which he was experimenting by a butterfly tattoo, and the alpha male wants her back. The alpha male makes a butterfly-shaped smear on the glass. He then takes a grenade and kills the Darkseekers and himself, saving the cure. Discovering that the last treatment was successful, Neville draws a vial of her blood and gives it to Anna, before shutting Ethan and her inside a coal chute in the back of the lab. Neville, Anna, and Ethan retreat into the basement laboratory, sealing themselves in with the female Darkseeker on which Neville has been experimenting. Neville once again attempts to administer a potential cure to the infected woman in his laboratory, but the next night, a group of Darkseekers, who had followed Anna and Neville back the night before, attacks the house and overruns its defenses. Neville angrily argues that no such survivor's camp exists. They take the injured Neville back to his home, where Anna explains that they survived the outbreak aboard a Red Cross evacuation ship from São Paulo and are making their way to a survivors' camp in Bethel, Vermont. He is nearly killed, but is rescued by a pair of immune survivors, Anna (Alice Braga) and a young boy named Ethan (Charlie Tahan), who have traveled from Maryland after hearing one of his broadcasts. The next night, driven over the edge by loneliness, he ventures out and suicidally attacks a group of Darkseekers. Neville brings Sam home and injects her with a strand of his serum, but when she shows signs of infection and tries to attack him, Neville is forced to strangle her to death. Sam and he manage to kill them, but Sam is bitten in the fight. The next day, he is ensnared in a trap similar to the one he used to capture the female, and by the time he manages to escape, it is dark and he is attacked by infected dogs. Back in his laboratory in the basement of his house, Neville treats the female without success. Neville finds a promising treatment derived from his own blood, so he sets a snare trap and captures a female Darkseeker while the alpha male Darkseeker watches from the shadows. ![]() Neville cautiously goes in after her and finds the deer's corpse along with Sam, but the building is infested by a colony of Darkseekers, both manage to escape unharmed and the attacking Darkseekers are killed by the sunlight. One day while waiting for survivors, Sam follows a deer into a dark building while she and Robert were hunting. At night, he barricades himself and Sam inside his heavily fortified Washington Square Park home to hide from the Darkseekers. Neville's loneliness is mitigated only by the companionship of his dog who is a German Shepherd, Sam, interaction with mannequins he has set up as patrons at a video store, and recordings of old television broadcasts. Flashbacks reveal that his wife (Salli Richardson) and daughter (Willow Smith) died in a helicopter accident during the chaotic evacuation of Manhattan, prior to the military-enforced quarantine of the island in 2009 with him and his dog as the sole survivors of the accident. He also waits each day for a response to his continuous recorded radio broadcasts, which instruct any uninfected survivors to meet him at midday at the South Street Seaport. Neville's daily routine includes experimentation on infected rats to find a cure for the virus and trips through Manhattan to hunt for food and supplies. The city has fallen into ruins and overgrown vegetation covers crumbling buildings and wild deer roam the streets while being preyed upon by lions that have escaped Central Park Zoo. Animals that coexist with humans like rats and domestic dogs are infected but wild animals like deer and lions are unaffected. The effects of the virus vary in other species. Three years after the outbreak, US Army virologist Lieutenant Colonel Robert Neville (Will Smith) lives an isolated life in the ruins of New York City, which is now deserted, unsure if any other uninfected humans are left in the world. In 2009, a genetically re-engineered measles virus, originally created as a cure for cancer, turns into a lethal strain which kills 90% of those it infects, and mutates the remaining 10% into predatory, nocturnal mutants called "Darkseekers" who are extremely vulnerable to sunlight and other sources of UV.
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